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KD/KO Validated

TPPII Polyclonal antibody

Name: TPPII Polyclonal antibody
Brand: Proteintech
SKU: 14120-1-AP

TPPII Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14120-1-AP

Synonyms

TPP2, tripeptidyl peptidase II, TPP-2, TPP II, TPP 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of Jurkat using 14120-1-AP
WB result of TPPII antibody (14120-1-AP, 1:1000) with si-control and si-TPPII transfected Jurkat cells.
WB analysis of Jurkat using 14120-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14120-1-AP (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14120-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14120-1-AP (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 14120-1-AP
IP result of anti-TPPII (IP:14120-1-AP, 4ug; Detection:66017-1-Ig, 1:500) with Jurkat cells lysate 2400ug.
IP experiment of Jurkat using 14120-1-AP
IP result of anti-TPPII (IP:14120-1-AP, 4ug; Detection:14120-1-AP 1:500) with Jurkat cells lysate 2400ug.
IHC staining of human gliomas using 14120-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14120-1-AP (TPPII antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14120-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14120-1-AP (TPPII antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14120-1-AP
Immunofluorescent analysis of HepG2 cells, using TPP2 antibody 14120-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
FC experiment of HepG2 using 14120-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TPPII Polyclonal antibody (14120-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 14120-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, Jurkat
Positive IP detected in	Jurkat cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IF	See 2 publications below

Product Information

14120-1-AP targets TPPII in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TPPII fusion protein Ag5296 相同性解析による交差性が予測される生物種
Full Name	tripeptidyl peptidase II
Calculated molecular weight	1249 aa, 138 kDa
Observed molecular weight	135-140 kDa
GenBank accession number	BC039905
Gene symbol	TPPII
Gene ID (NCBI)	7174
RRID	AB_2206028
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

Protocols

Product Specific Protocols
WB protocol for TPPII antibody 14120-1-AP	Download protocol
IHC protocol for TPPII antibody 14120-1-AP	Download protocol
IF protocol for TPPII antibody 14120-1-AP	Download protocol
IP protocol for TPPII antibody 14120-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Sci Unveiling a novel serpinB2-tripeptidyl peptidase II signaling axis during senescence. Authors - Chia-Li Liao KD Validated View Article
human	WB	J Immunol Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen. Authors - Nathalie Vigneron View Article
human	IF	Mol Cell Biochem Novel protein-protein interactions of TPPII, p53, and SIRT7. Authors - Jarmila Nahálková View Article
human	WB,IF	Genes Cancer Tripeptidyl Peptidase II Is Required for c-MYC-Induced Centriole Overduplication and a Novel Therapeutic Target in c-MYC-Associated Neoplasms. Authors - Stefan Duensing KD Validated View Article
human	WB	J Immunol The Activation State of CD4 T Cells Alters Cellular Peptidase Activities, HIV Antigen Processing, and MHC Class I Presentation in a Sequence-Dependent Manner. Authors - Julie Boucau View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPPII Polyclonal antibody

TPPII Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 14120-1-AP

WB analysis of Jurkat using 14120-1-AP

WB analysis of K-562 using 14120-1-AP

IP experiment of Jurkat using 14120-1-AP

IP experiment of Jurkat using 14120-1-AP

IHC staining of human gliomas using 14120-1-AP

IHC staining of human gliomas using 14120-1-AP

IF Staining of HepG2 using 14120-1-AP

FC experiment of HepG2 using 14120-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Unveiling a novel serpinB2-tripeptidyl peptidase II signaling axis during senescence.

Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen.

Novel protein-protein interactions of TPPII, p53, and SIRT7.

Tripeptidyl Peptidase II Is Required for c-MYC-Induced Centriole Overduplication and a Novel Therapeutic Target in c-MYC-Associated Neoplasms.

The Activation State of CD4 T Cells Alters Cellular Peptidase Activities, HIV Antigen Processing, and MHC Class I Presentation in a Sequence-Dependent Manner.