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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of Jurkat using 66017-1-Ig (same clone as 66017-1-PBS)

    WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of HepG2 using 66017-1-Ig (same clone as 66017-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of Raji using 66017-1-Ig (same clone as 66017-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TPPII Monoclonal antibody (66017-1-Ig)

    IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TPPII Monoclonal antibody (66017-1-Ig)

    IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TPPII Monoclonal antibody (66017-1-Ig)

    IF Staining of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TPPII Monoclonal antibody (66017-1-Ig)

    IF Staining of MCF-7 using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TPPII Monoclonal antibody, PBS Only

TPPII Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66017-1-PBS
Clone No.5H6F11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66017-1-PBS targets TPPII in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPPII fusion protein Ag5296 相同性解析による交差性が予測される生物種
Full Name tripeptidyl peptidase II
Calculated molecular weight 1249 aa, 138 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC039905
Gene Symbol TPPII
Gene ID (NCBI) 7174
RRIDAB_11044195
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29144
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).