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  • Featured Product
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TPPP Monoclonal antibody

TPPP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E5F5

Cat no : 68048-1-Ig

Synonyms

p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis using 68048-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of SH-SY5Y cells using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis of SH-SY5Y using 68048-1-Ig

    WB result of TPPP antibody (68048-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells.

  • Western Blot (WB) analysis of pig cerebellum tissue using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis of pig cerebellum using 68048-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit cerebellum tissue using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis of rabbit cerebellum using 68048-1-Ig

    rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis of rat cerebellum using 68048-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TPPP Monoclonal antibody (68048-1-Ig)

    IHC staining of mouse brain using 68048-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using TPPP Monoclonal antibody (68048-1-Ig)

    IHC staining of human lung using 68048-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using TPPP Monoclonal antibody (68048-1-Ig)

    IHC staining of human lung using 68048-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TPPP Monoclonal antibody (68048-1-Ig)

    IHC staining of mouse brain using 68048-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, SH-SY5Y cells, rabbit brain, rat brain, mouse brain, chicken brain
Positive IHC detected inhuman lung tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68048-1-Ig targets TPPP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPPP fusion protein Ag19046 相同性解析による交差性が予測される生物種
Full Name tubulin polymerization promoting protein
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight24-29 kDa
GenBank accession numberBC131506
Gene symbol TPPP
Gene ID (NCBI) 11076
RRIDAB_2918790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.

Protocols

Product Specific Protocols
WB protocol for TPPP antibody 68048-1-IgDownload protocol
IHC protocol for TPPP antibody 68048-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols