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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPRKB Monoclonal antibody

TPRKB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5B9

Cat no : 66402-1-Ig

Synonyms

TP53RK-binding protein, PRPK-binding protein, My019, EKC/KEOPS complex subunit TPRKB, CGI-121

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TPRKB Monoclonal antibody (66402-1-Ig)

    WB analysis of HeLa using 66402-1-Ig

    Western blot analysis of TPRKB in various cell lines using Proteintech antibody 66402-1-Ig at a dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TPRKB Monoclonal antibody (66402-1-Ig)

    WB analysis using 66402-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66402-1-Ig (TPRKB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TPRKB Monoclonal antibody (66402-1-Ig)

    WB analysis of HEK-293 using 66402-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66402-1-Ig (TPRKB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TPRKB Monoclonal antibody (66402-1-Ig)

    IHC staining of human skin cancer using 66402-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66402-1-Ig (TPRKB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using TPRKB Monoclonal antibody (66402-1-Ig)

    IF Staining of Jurkat using 66402-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TPRKB antibody (66402-1-Ig, Clone: 2D5B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, Caco-2 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66402-1-Ig targets TPRKB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPRKB fusion protein Ag18770 相同性解析による交差性が予測される生物種
Full Name TP53RK binding protein
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC029492
Gene symbol TPRKB
Gene ID (NCBI) 51002
RRIDAB_2881776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPRKB, also named as CGI 121, belongs to the CGI121/TPRKB family. It interacts with TP53RK/PRPK and is widely expressed in all tissues.

Protocols

Product Specific Protocols
WB protocol for TPRKB antibody 66402-1-IgDownload protocol
IHC protocol for TPRKB antibody 66402-1-IgDownload protocol
IF protocol for TPRKB antibody 66402-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols