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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPSAB1 Polyclonal antibody (13343-1-AP)

    WB analysis using 13343-1-AP (same clone as 13343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using TPSAB1 Polyclonal antibody (13343-1-AP)

    WB analysis of A431 using 13343-1-AP (same clone as 13343-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IP experiment of mouse liver using 13343-1-AP (same clone as 13343-1-PBS)

    IP result of anti-TPSAB1 (IP:13343-1-AP, 4ug; Detection:13343-1-AP 1:800) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendix tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human appendix using 13343-1-AP (same clone as 13343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendix using 13343-1-AP (TPSAB1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using TPSAB1 Polyclonal antibody (13343-1-AP)

    IF Staining of mouse skin using 13343-1-AP (same clone as 13343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TPSAB1 antibody (13343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPSAB1 Polyclonal antibody (13343-1-AP)

    IF Staining of HepG2 using 13343-1-AP (same clone as 13343-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13343-1-AP (TPSAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-937 cells using TPSAB1 Polyclonal antibody (13343-1-AP)

    FC experiment of U-937 using 13343-1-AP (same clone as 13343-1-PBS)

    1X10^6 U-937 cells were intracellularly stained with 0.2 ug Anti-Human TPSAB1 (13343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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TPSAB1 Polyclonal antibody, PBS Only

TPSAB1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 13343-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13343-1-PBS targets TPSAB1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPSAB1 fusion protein Ag4167 相同性解析による交差性が予測される生物種
Full Name tryptase alpha/beta 1
Calculated molecular weight 275 aa, 31 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberBC028059
Gene Symbol TPSAB1
Gene ID (NCBI) 7177
RRIDAB_2206197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15661
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.