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  • Featured Product
  • KD/KO Validated

TPT1 Monoclonal antibody, PBS Only

TPT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6A10

Cat no : 66713-1-PBS

Synonyms

TCTP, p23, HRF, Histamine-releasing factor, Fortilin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPT1 Monoclonal antibody (66713-1-Ig)

    WB analysis using 66713-1-Ig (same clone as 66713-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TPT1 Monoclonal antibody (66713-1-Ig)

    WB analysis of MCF-7 using 66713-1-Ig (same clone as 66713-1-PBS)

    WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TPT1 Monoclonal antibody (66713-1-Ig)

    WB analysis of HeLa using 66713-1-Ig (same clone as 66713-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using TPT1 Monoclonal antibody (66713-1-Ig)

    WB analysis of RAW 264.7 using 66713-1-Ig (same clone as 66713-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPT1 Monoclonal antibody (66713-1-Ig)

    IHC staining of human colon cancer using 66713-1-Ig (same clone as 66713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPT1 Monoclonal antibody (66713-1-Ig)

    IHC staining of human colon cancer using 66713-1-Ig (same clone as 66713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TPT1 Monoclonal antibody (66713-1-Ig)

    IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TPT1 Monoclonal antibody (66713-1-Ig)

    IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66713-1-PBS targets TPT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPT1 fusion protein Ag27651 相同性解析による交差性が予測される生物種
Full Name tumor protein, translationally-controlled 1
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003352
Gene symbol TPT1
Gene ID (NCBI) 7178
RRIDAB_2882064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.