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  • Featured Product
  • KD/KO Validated

TPX2 Polyclonal antibody, PBS Only

TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11741-1-PBS

Synonyms

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPX2 Polyclonal antibody (11741-1-AP)

    WB analysis using 11741-1-AP (same clone as 11741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using TPX2 Polyclonal antibody (11741-1-AP)

    WB analysis of K-562 using 11741-1-AP (same clone as 11741-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TPX2 Polyclonal antibody (11741-1-AP)

    IP experiment of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    IP result of anti-TPX2 (IP:11741-1-AP, 4ug; Detection:11741-1-AP 1:800) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human prostate cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11741-1-AP (TPX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human breast cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human breast cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TPX2 Polyclonal antibody (11741-1-AP)

    IF Staining of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPX2 Polyclonal antibody (11741-1-AP)

    IF Staining of HepG2 using 11741-1-AP (same clone as 11741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (11741-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TPX2 Polyclonal antibody (11741-1-AP)

    IF Staining of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11741-1-PBS targets TPX2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPX2 fusion protein Ag2334 相同性解析による交差性が予測される生物種
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight 747 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC020207
Gene symbol TPX2
Gene ID (NCBI) 22974
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).