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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPX2 Monoclonal antibody

TPX2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E10C1

Cat no : 68712-1-Ig

Synonyms

C20orf1, C20orf2, DIL 2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, p100, Protein fls353, REPP86, Targeting protein for Xklp2, TPX2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPX2 Monoclonal antibody (68712-1-Ig)

    WB analysis using 68712-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TPX2 Monoclonal antibody (68712-1-Ig)

    WB analysis using 68712-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TPX2 Monoclonal antibody (68712-1-Ig)

    WB analysis of HEK-293 using 68712-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TPX2 Monoclonal antibody (68712-1-Ig)

    WB analysis of Jurkat using 68712-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, HeLa cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68712-1-Ig targets TPX2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPX2 fusion protein Ag33683 相同性解析による交差性が予測される生物種
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight 747 aa, 86 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC020207
Gene symbol TPX2
Gene ID (NCBI) 22974
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

Protocols

Product Specific Protocols
WB protocol for TPX2 antibody 68712-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols