TPX2 Monoclonal antibody
TPX2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2E10C1
Cat no : 68712-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, HeLa cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68712-1-Ig targets TPX2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TPX2 fusion protein Ag33683 相同性解析による交差性が予測される生物種 |
| Full Name | TPX2, microtubule-associated, homolog (Xenopus laevis) |
| Calculated molecular weight | 747 aa, 86 kDa |
| Observed molecular weight | 98 kDa |
| GenBank accession number | BC020207 |
| Gene symbol | TPX2 |
| Gene ID (NCBI) | 22974 |
| RRID | AB_3670405 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TPX2 antibody 68712-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
