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  • Featured Product
  • KD/KO Validated

TRA2B Polyclonal antibody

TRA2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29627-1-AP

Synonyms

TRA 2 beta, SRFS10, Splicing factor, arginine/serine-rich 10, SFRS10, hTRA2-beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRA2B Polyclonal antibody (29627-1-AP)

    WB analysis using 29627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29627-1-AP (TRA2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TRA2B Polyclonal antibody (29627-1-AP)

    WB analysis using 29627-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 29627-1-AP (TRA2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using TRA2B Polyclonal antibody (29627-1-AP)

    IHC staining of human stomach using 29627-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29627-1-AP (TRA2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29627-1-AP targets TRA2B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRA2B fusion protein Ag30637 相同性解析による交差性が予測される生物種
Full Name transformer 2 beta homolog (Drosophila)
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC005898
Gene symbol TRA2B
Gene ID (NCBI) 6434
RRIDAB_2918335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transformer-2 protein homolog beta, also known as TRA2B, is a sequence-specific RNA-binding protein that participates in the control of pre-mRNA splicing. TRA2B can alter pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX.

Protocols

Product Specific Protocols
WB protocol for TRA2B antibody 29627-1-APDownload protocol
IHC protocol for TRA2B antibody 29627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biomed Environ Sci

Tra2β Enhances Cell Proliferation by Inducing the Expression of Transcription Factor SP1 in Cervical Cancer

Authors - Mo Juan Li
  • KD Validated
View Article