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KD/KO Validated

TRAF2 Polyclonal antibody

Name: TRAF2 Polyclonal antibody
Brand: Proteintech
SKU: 26846-1-AP

TRAF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26846-1-AP

Synonyms

TRAF2, TRAP, TRAP3

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HepG2 using 26846-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26846-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 26846-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 26846-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 26846-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 26846-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 26846-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of A549 using 26846-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRAF2 antibody (26846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 26846-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26846-1-AP (TRAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HUVEC using 26846-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 26846-1-AP (TRAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 26846-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, human testis tissue, HeLa cells
Positive IHC detected in	human liver cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells, HeLa cells, HUVEC cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 28 publications below
IHC	See 4 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

26846-1-AP targets TRAF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRAF2 fusion protein Ag25387 相同性解析による交差性が予測される生物種
Full Name	TNF receptor-associated factor 2
Calculated molecular weight	56 kDa
Observed molecular weight	53 kDa
GenBank accession number	BC032410
Gene symbol	TRAF2
Gene ID (NCBI)	7186
RRID	AB_2880656
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TRAF2 antibody 26846-1-AP	Download protocol
IHC protocol for TRAF2 antibody 26846-1-AP	Download protocol
IF protocol for TRAF2 antibody 26846-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response Authors - Zhe Wang View Article
human	IHC	J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. Authors - Ning Xu View Article
mouse	WB	J Hazard Mater Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells. Authors - Qiushuang Zhu View Article
human	WB	EMBO Rep Snail enhances arginine synthesis by inhibiting ubiquitination-mediated degradation of ASS1. Authors - Hao Jia KD Validated View Article
human,mouse	WB,IHC,IF	Free Radic Biol Med TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis Authors - Jiping Yao KD Validated View Article
mouse	IF	J Nanobiotechnology The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation. Authors - Yanglin Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAF2 Polyclonal antibody

TRAF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 26846-1-AP

WB analysis of HeLa using 26846-1-AP

WB analysis of human testis using 26846-1-AP

IHC staining of human liver cancer using 26846-1-AP

IHC staining of human liver cancer using 26846-1-AP

IHC staining of human kidney using 26846-1-AP

IHC staining of human kidney using 26846-1-AP

IF Staining of A549 using 26846-1-AP

IF Staining of HeLa using 26846-1-AP

IF Staining of HUVEC using 26846-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.

Snail enhances arginine synthesis by inhibiting ubiquitination-mediated degradation of ASS1.

TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis

The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation.