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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAF2 Recombinant antibody, PBS Only

TRAF2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241899A2

Cat no : 84581-3-PBS

Synonyms

TRAP, TNF receptor-associated factor 2, RING-type E3 ubiquitin transferase TRAF2, EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAF2 Recombinant antibody (84581-3-RR)

    WB analysis using 84581-3-RR (same clone as 84581-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84581-3-RR (TRAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84581-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAF2 Recombinant antibody (84581-3-RR)

    IF Staining of HeLa using 84581-3-RR (same clone as 84581-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (84581-3-RR, Clone: 241899A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84581-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84581-3-RR

    Affinity and Kinetic Characterization of 84581-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84581-3-RR against Human TRAF2 were performed. The affinity constant is 0.544 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84581-3-PBS targets TRAF2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRAF2 fusion protein Ag25387 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 2
Calculated molecular weight 56 kDa
Observed molecular weight53 kDa
GenBank accession numberBC032410
Gene symbol TRAF2
Gene ID (NCBI) 7186
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tumor necrosis factor (TNF) receptor-associated factor-2 (TRAF2) is one of the members of the TRAF superfamily protein, which is an intracellular junction protein with E3 ligase activity. TRAF2 mediates and regulates the activation of nuclear factor kappa B (NF-κB) and microtubule-associated protein kinase (MAPK) signaling pathways by binding to TNFR family proteins. Recent studies have demonstrated that TRAF2 regulates tumor progression by through multiple pathways.