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  • Featured Product
  • KD/KO Validated

TRAF3 Monoclonal antibody, PBS Only

TRAF3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3F4

Cat no : 66310-1-PBS

Synonyms

CD40 receptor-associated factor 1, CD40 binding protein, CAP-1, CAP1, CAP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAF3 Monoclonal antibody (66310-1-Ig)

    WB analysis using 66310-1-Ig (same clone as 66310-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66310-1-Ig (TRAF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TRAF3 Monoclonal antibody (66310-1-Ig)

    WB analysis of HEK-293 using 66310-1-Ig (same clone as 66310-1-PBS)

    WB result of TRAF3 antibody (66310-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAF3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRAF3 Monoclonal antibody (66310-1-Ig)

    WB analysis using 66310-1-Ig (same clone as 66310-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66310-1-Ig (TRAF3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using TRAF3 Monoclonal antibody (66310-1-Ig)

    WB analysis of U-937 using 66310-1-Ig (same clone as 66310-1-PBS)

    LPS treated U-937 cells were subjected to SDS PAGE followed by western blot with 66310-1-Ig (TRAF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using TRAF3 Monoclonal antibody (66310-1-Ig)

    IHC staining of human lymphoma using 66310-1-Ig (same clone as 66310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66310-1-Ig (TRAF3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using TRAF3 Monoclonal antibody (66310-1-Ig)

    IHC staining of human lymphoma using 66310-1-Ig (same clone as 66310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66310-1-Ig (TRAF3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAF3 Monoclonal antibody (66310-1-Ig)

    IF Staining of HeLa using 66310-1-Ig (same clone as 66310-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF3 antibody (66310-1-Ig, Clone: 1E3F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66310-1-PBS targets TRAF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TRAF3 fusion protein Ag12185 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 3
Calculated molecular weight 568 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC075087
Gene symbol TRAF3
Gene ID (NCBI) 7187
RRIDAB_2881692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5.