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KD/KO Validated

TRAF3IP2 Polyclonal antibody

Name: TRAF3IP2 Polyclonal antibody
Brand: Proteintech
SKU: 26692-1-AP

TRAF3IP2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26692-1-AP

Synonyms

C6orf6, C6orf5, C6orf4, C6orf2, Adapter protein CIKS

Validation Data Gallery

Filter:

AllWBIF/ICCFC (Intra)

WB analysis of MCF-7 using 26692-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 26692-1-AP (TRAF3IP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 26692-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 26692-1-AP (TRAF3IP2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of A549 using 26692-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 26692-1-AP (TRAF3IP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 26692-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26692-1-AP (TRAF3IP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 26692-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TRAF3IP2 Polyclonal antibody (26692-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 26692-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, A549 cells, HepG2 cells
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below

Product Information

26692-1-AP targets TRAF3IP2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRAF3IP2 fusion protein Ag24840 相同性解析による交差性が予測される生物種
Full Name	TRAF3 interacting protein 2
Calculated molecular weight	65 kDa
Observed molecular weight	65 kDa
GenBank accession number	BC002823
Gene symbol	TRAF3IP2
Gene ID (NCBI)	10758
RRID	AB_2880606
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TRAF3IP2 antibody 26692-1-AP	Download protocol
IF protocol for TRAF3IP2 antibody 26692-1-AP	Download protocol
FC protocol for TRAF3IP2 antibody 26692-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	WB	Oxid Med Cell Longev Minocycline Alleviates White Matter Injury following Intracerebral Hemorrhage by Regulating CD4+ T Cell Differentiation via Notch1 Signaling Pathway. Authors - Heng Yang View Article
mouse	WB	Arch Toxicol Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes. Authors - Yangfei Zhao View Article
human,mouse	WB	Ecotoxicol Environ Saf Hexavalent chromium triggers hepatocytes premature senescence via the GATA4/NF-κB signaling pathway mediated by the DNA damage response. Authors - Yu Ma View Article
human	WB	Oxid Med Cell Longev Prognostic Assessment of Oxidative Stress-Related Genes in Colorectal Cancer and New Insights into Tumor Immunity Authors - Zilu Chen KD Validated View Article
human	WB	Heliyon TRAF3IP2 drives mesenchymal stem cell senescence via regulation of NAMPT-mediated NAD biosynthesis Authors - Xiaoran Huang KD Validated View Article
mouse,pig	WB	Cell Rep Comparing HD knockin pigs and mice reveals the pathological role of IL-17 Authors - Qingqing Jia View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAF3IP2 Polyclonal antibody

TRAF3IP2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of MCF-7 using 26692-1-AP

WB analysis of HepG2 using 26692-1-AP

WB analysis of A549 using 26692-1-AP

IF Staining of HepG2 using 26692-1-AP

FC experiment of HepG2 using 26692-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Minocycline Alleviates White Matter Injury following Intracerebral Hemorrhage by Regulating CD4+ T Cell Differentiation via Notch1 Signaling Pathway.

Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes.

Hexavalent chromium triggers hepatocytes premature senescence via the GATA4/NF-κB signaling pathway mediated by the DNA damage response.

Prognostic Assessment of Oxidative Stress-Related Genes in Colorectal Cancer and New Insights into Tumor Immunity

TRAF3IP2 drives mesenchymal stem cell senescence via regulation of NAMPT-mediated NAD biosynthesis

Comparing HD knockin pigs and mice reveals the pathological role of IL-17