Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, PC-3 cells, mouse pancreas tissue, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 2 publications below |
Product Information
12868-1-AP targets TRAF5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRAF5 fusion protein Ag3519 相同性解析による交差性が予測される生物種 |
| Full Name | TNF receptor-associated factor 5 |
| Calculated molecular weight | 557 aa, 64 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC029600 |
| Gene Symbol | TRAF5 |
| Gene ID (NCBI) | 7188 |
| RRID | AB_2209712 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00463 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF5 antibody 12868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neuroinflammation CD36 deletion prevents white matter injury by modulating microglia polarization through the Traf5-MAPK signal pathway | ||
Cancer Lett Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells. | ||
Biochim Biophys Acta Mol Cell Res IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5 | ||
J Cell Mol Med Down-regulated HDAC3 elevates microRNA-495-3p to restrain epithelial-mesenchymal transition and oncogenicity of melanoma cells via reducing TRAF5.
| ||
Eur J Pharmacol TRAF5 protects against myocardial ischemia reperfusion injury via AKT signaling.
| ||
Med Oncol DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. |