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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TRAF5 Monoclonal antibody (66991-1-Ig)

    WB analysis of HeLa using 66991-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TRAF5 Monoclonal antibody (66991-1-Ig)

    WB analysis of PC-3 using 66991-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using TRAF5 Monoclonal antibody (66991-1-Ig)

    WB analysis of LNCaP using 66991-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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TRAF5 Monoclonal antibody

TRAF5 Monoclonal Antibody for WB, ELISA
Cat No. 66991-1-Ig
Clone No.1B1A7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

RING finger protein 84, RNF84, TRAF5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66991-1-Ig targets TRAF5 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAF5 fusion protein Ag26070 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 5
Calculated molecular weight 557 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC029600
Gene Symbol TRAF5
Gene ID (NCBI) 7188
RRIDAB_2882308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00463
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAF5 (also known as TNF receptor-associated factor 5, Ring finger protein 84, RNF8, MGC:39780) is a member of the tumor necrosis factor receptor-associated (TRAF) family of proteins that are characterized by the presence of a meprin and TRAF homology (MATH) domain, a RING-type zinc finger domain, and two TRAF-type zinc finger domains (https://www.uniprot.org/uniprot/O00463). TRAF5 is a scaffold protein that associates with and mediates signal transduction by cell surface receptors belonging to the tumor necrosis factor (TNF) superfamily, including CD27, CD30, CD40, and lymphotoxin-β receptor, positively regulating activation of the canonical NF-κB pathway and c-Jun NH2-terminal kinase (JNK) activation by these receptors (PMIDs: 8999898, 9582383, 8790348, 8663299). The RING-type zinc finger domain of TRAF5 exhibits ubiquitin protein ligase activity and is responsible for Lys-63-linked polyubiquitination of protein targets (PMIDs:23758787, 29176576). This domain has been demonstrated to be required for TRAF5-mediated activation of NF-κB (PMID:8663299).

Protocols

Product Specific Protocols
WB protocol for TRAF5 antibody 66991-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols