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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAF6 Monoclonal antibody (66498-1-Ig)

    WB analysis using 66498-1-Ig (same clone as 66498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRAF6 Monoclonal antibody (66498-1-Ig)

    WB analysis using 66498-1-Ig (same clone as 66498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRAF6 Monoclonal antibody (66498-1-Ig)

    IHC staining of human liver using 66498-1-Ig (same clone as 66498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRAF6 Monoclonal antibody (66498-1-Ig)

    IHC staining of human liver using 66498-1-Ig (same clone as 66498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TRAF6 Monoclonal antibody (66498-1-Ig)

    IHC staining of human heart using 66498-1-Ig (same clone as 66498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TRAF6 Monoclonal antibody (66498-1-Ig)

    IHC staining of human heart using 66498-1-Ig (same clone as 66498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAF6 Monoclonal antibody (66498-1-Ig)

    IF Staining of HeLa using 66498-1-Ig (same clone as 66498-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF6 antibody (66498-1-Ig, Clone: 1D1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TRAF6 Monoclonal antibody, PBS Only

TRAF6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66498-1-PBS
Clone No.1D1E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RNF85, RING-type E3 ubiquitin transferase TRAF6, Interleukin-1 signal transducer, EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF6

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66498-1-PBS targets TRAF6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAF6 fusion protein Ag24696 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 6
Calculated molecular weight 522 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC031052
Gene Symbol TRAF6
Gene ID (NCBI) 7189
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4K3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.