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- KD/KO Validated
TRAF7 Polyclonal antibody
TRAF7 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11780-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
11780-1-AP targets TRAF7 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRAF7 fusion protein Ag2414 相同性解析による交差性が予測される生物種 |
| Full Name | TNF receptor-associated factor 7 |
| Calculated molecular weight | 75 kDa |
| Observed molecular weight | 67-75 kDa |
| GenBank accession number | BC024267 |
| Gene symbol | TRAF7 |
| Gene ID (NCBI) | 84231 |
| RRID | AB_2877793 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF7 antibody 11780-1-AP | Download protocol |
| IHC protocol for TRAF7 antibody 11780-1-AP | Download protocol |
| IP protocol for TRAF7 antibody 11780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Kidney Int Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney. | ||
Mol Ther Nucleic Acids HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis. | ||
Virol Sin TRAF7 negatively regulates the RLR signaling pathway by facilitating the K48-linked ubiquitination of TBK1
| ||
Cell Death Discov TRAF7-targeted HOXA5 acts as a tumor suppressor in prostate cancer progression and stemness via transcriptionally activating SPRY2 and regulating MEK/ERK signaling | ||
Free Radic Biol Med Hydrogen sulfide improves endothelial barrier function by modulating the ubiquitination degradation of KLF4 through TRAF7 S-sulfhydration in diabetic aorta | ||
Genes (Basel) Expression of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) Candidate Genes EDA2R, PCDH9, and TRAF7 in Normal Human Kidney Development and CAKUT |
