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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAIL Monoclonal antibody, PBS Only (Capture)

TRAIL Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9B4

Cat no : 66756-1-PBS

Synonyms

TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253

Validation Data Gallery

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  • Western Blot (WB) analysis of human milk using TRAIL Monoclonal antibody (66756-1-Ig)

    WB analysis of human milk using 66756-1-Ig (same clone as 66756-1-PBS)

    human milk were subjected to SDS PAGE followed by western blot with 66756-1-Ig (TRAIL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TRAIL Monoclonal antibody (66756-1-Ig)

    IHC staining of human tonsillitis using 66756-1-Ig (same clone as 66756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TRAIL Monoclonal antibody (66756-1-Ig)

    IHC staining of human tonsillitis using 66756-1-Ig (same clone as 66756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TRAIL Monoclonal antibody (66756-1-Ig)

    IHC staining of human prostate cancer using 66756-1-Ig (same clone as 66756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TRAIL Monoclonal antibody (66756-1-Ig)

    IHC staining of human prostate cancer using 66756-1-Ig (same clone as 66756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50341-1

    Cytometric bead array standard curve of MP50341-1

    Cytometric bead array standard curve of MP50341-1, TRAIL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66756-1-PBS. Detection antibody: 66756-2-PBS. Standard:Ag25746. Range: 3.125-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66756-1-PBS targets TRAIL as part of a matched antibody pair:

MP50341-1: 66756-1-PBS capture and 66756-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAIL fusion protein Ag25746 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 10
Calculated molecular weight 281 aa, 33 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC032722
Gene symbol TRAIL
Gene ID (NCBI) 8743
RRIDAB_2882102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNFSF10/TRAIL (tumor necrosis factor superfamily member 10) is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TNFSF10 induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.