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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAIL Recombinant antibody, PBS Only (Detector)

TRAIL Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241419C12

Cat no : 84280-1-PBS

Synonyms

TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAIL Recombinant antibody (84280-1-RR)

    WB analysis using 84280-1-RR (same clone as 84280-1-PBS)

    Human milk samples were subjected to SDS PAGE followed by western blot with 84280-1-RR (TRAIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84280-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01166-1

    Sandwich ELISA standard curve of MP01166-1

    Sandwich ELISA standard curve of MP01166-1, Human TRAIL Recombinant Matched Antibody Pair - PBS only. 84280-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02339. 84280-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL

  • Sample test of MP01166-1

    Sample test of MP01166-1

    Serum of six individual healthy human donors was measured. The human TRAIL concentration of detected samples was determined to be 182.8 pg/mL with a range of 33.7 - 368.3 pg/mL.

  • Sample test of MP01166-1

    Sample test of MP01166-1

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean TRAIL concentration was undetectable in unstimulated PBMC supernatant, 22.3 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean TRAIL concentration was determined to be 1.0 pg/mL in unstimulated PBMC supernatant, 22.9 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01166-1

    Sample test of MP01166-1

    Saliva of six individual healthy human donors was measured. The human TRAIL concentration of detected samples was determined to be 809.9 pg/mL with a range of 159.6 - 1,340.0 pg/mL.

  • Cytometric bead array standard curve of MP01166-1

    Cytometric bead array standard curve of MP01166-1

    Cytometric bead array standard curve of MP01166-1, TRAIL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84280-2-PBS. Detection antibody: 84280-1-PBS. Standard: RP02339. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84280-1-PBS targets TRAIL as part of a matched antibody pair:

MP01166-1: 84280-2-PBS capture and 84280-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 10
Calculated molecular weight 281 aa, 33 kDa
Observed molecular weight22-25 kDa
GenBank accession numberBC032722
Gene symbol TRAIL
Gene ID (NCBI) 8743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.