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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood mononuclear cells using Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR)

    FC experiment of human peripheral blood mononuclear cells using 98139-1-RR

    1x10^6 untreated or IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood mononuclear cells using Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR)

    FC experiment of human peripheral blood mononuclear cells using 98139-1-RR

    1x10^6 IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98139-1-RR

    Affinity and Kinetic Characterization of 98139-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98139-1-RR against Human TRAIL/CD253 were performed. The affinity constant is below 1 pM.

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Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody

TRAIL/CD253 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98139-1-RR
Clone No.241419D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TNFSF10, TRAIL, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inIFN-alpha treated human peripheral blood mononuclear cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98139-1-RR targets TRAIL/CD253 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 10
Calculated molecular weight 281 aa, 33 kDa
GenBank accession numberBC032722
Gene Symbol TRAIL
Gene ID (NCBI) 8743
RRIDAB_3672285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50591
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.

Protocols

Product Specific Protocols
FC protocol for TRAIL/CD253 antibody 98139-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols