TRAM1 Polyclonal antibody

Name: TRAM1 Polyclonal antibody
Brand: Proteintech
SKU: 12705-1-AP

TRAM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12705-1-AP

Synonyms

Translocating chain-associated membrane protein 1, TRAM, Protein TRAM1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of K-562 using 12705-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12705-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12705-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12705-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 12705-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12705-1-AP (TRAM1 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of PC-3 using 12705-1-AP
Immunofluorescent analysis of PC-3 cells using 12705-1-AP (TRAM1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 12705-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, HeLa cells, Jurkat cells, PC-3 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

12705-1-AP targets TRAM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRAM1 fusion protein Ag3384 相同性解析による交差性が予測される生物種
Full Name	translocation associated membrane protein 1
Calculated molecular weight	374 aa, 43 kDa
Observed molecular weight	43 kDa
GenBank accession number	BC037738
Gene symbol	TRAM1
Gene ID (NCBI)	23471
RRID	AB_2282651
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TRAM1 antibody 12705-1-AP	Download protocol
IHC protocol for TRAM1 antibody 12705-1-AP	Download protocol
IF protocol for TRAM1 antibody 12705-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Front Pharmacol Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway Authors - Sairong Fan View Article
rat	WB	Evid Based Complement Alternat Med The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota. Authors - Boxun Zhang View Article
human	WB	Mol Cell An ATP13A1-assisted topogenesis pathway for folding multi-spanning membrane proteins Authors - Jia Ji View Article
human	WB	Front Endocrinol (Lausanne) Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis Authors - Han Chen View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAM1 Polyclonal antibody

TRAM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of K-562 using 12705-1-AP

WB analysis of HeLa using 12705-1-AP

WB analysis of Jurkat using 12705-1-AP

WB analysis of PC-3 using 12705-1-AP

IHC staining of human prostate cancer using 12705-1-AP

IF Staining of PC-3 using 12705-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota.

An ATP13A1-assisted topogenesis pathway for folding multi-spanning membrane proteins

Identification of immune-related endoplasmic reticulum stress genes in proliferative diabetic retinopathy using bioinformatics analysis