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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRANK1 Polyclonal antibody

TRANK1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27583-1-AP

Synonyms

KIAA0342, LBA1, lupus brain antigen 1, Lupus brain antigen 1 homolog, TRANK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRANK1 Polyclonal antibody (27583-1-AP)

    WB analysis using 27583-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27583-1-AP (TRANK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TRANK1 Polyclonal antibody (27583-1-AP)

    IHC staining of human ovary cancer using 27583-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27583-1-AP (TRANK1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27583-1-AP targets TRANK1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRANK1 fusion protein Ag26206 相同性解析による交差性が予測される生物種
Full Name lupus brain antigen 1
Calculated molecular weight 336 kDa
Observed molecular weight~330 kDa
GenBank accession numberNM_014831
Gene symbol LBA1
Gene ID (NCBI) 9881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Etratricopeptide repeat and ankyrin repeat containing 1 (TRANK1), has been confirmed as the susceptibility gene for BD (bipolar disorder). An integrated analysis of mRNA co-expression networks revealed that genes highly correlated with TRANK1 were significantly involved in the biological processes related to synaptic plasticity, dendritic spine, axon guidance and circadian rhythm. In addition, TRANK1 rs71947856 variant was associated with circadian regulation and Kleine-Levin syndrome, which is a rare disease characterized by severe episodic hypersomnia, with cognitive impairment and apathy or disinhibition.(PMID: 34014031)

Protocols

Product Specific Protocols
WB protocol for TRANK1 antibody 27583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols