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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAPPC10 Polyclonal antibody

TRAPPC10 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27549-1-AP

Synonyms

EHOC 1, EHOC1, GT334, Protein GT334, TMEM1, TRAPP subunit TMEM1, TRAPPC10, TRS130, TRS30

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPPC10 Polyclonal antibody (27549-1-AP)

    WB analysis using 27549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27549-1-AP (TRAPPC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TRAPPC10 Polyclonal antibody (27549-1-AP)

    IP experiment of mouse brain using 27549-1-AP

    IP result of anti-TRAPPC10 (IP:27549-1-AP, 4ug; Detection:27549-1-AP 1:600) with mouse brain tissue lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRAPPC10 Polyclonal antibody (27549-1-AP)

    IHC staining of mouse brain using 27549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using TRAPPC10 Polyclonal antibody (27549-1-AP)

    IHC staining of rat brain using 27549-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27549-1-AP targets TRAPPC10 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAPPC10 fusion protein Ag26900 相同性解析による交差性が予測される生物種
Full Name trafficking protein particle complex 10
Calculated molecular weight 1259 aa, 142 kDa
Observed molecular weight 142 kDa
GenBank accession numberBC101728
Gene symbol TRAPPC10
Gene ID (NCBI) 7109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAPPC10 (Trafficking protein particle complex subunit 10) is also named as EHOC-1, Protein GT334, TRAPP subunit TMEM1 and TMEM1. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. TRAPPC10 is specific component of the multisubunit TRAPP II complex (PMID: 23511850). TRAPPC10 can interacts with TRAPPC14 (PMID:31467083). TRAPPC10 interacts with TRAPPC2L in the TRAPP II complex (PMID: 30120216). TRAPPC10 variants are associated with a microcephalic neurodevelopmental disorder (PMID: 35298461). A new Longin at the N-terminus of human TRAPPC10 may dimerize with TRAPPC2 (PMID: 23511850). Therefore, a band with a high molecular weight of 150 - 250 kDa can be formed.

Protocols

Product Specific Protocols
IHC protocol for TRAPPC10 antibody 27549-1-APDownload protocol
IP protocol for TRAPPC10 antibody 27549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols