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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPPC11 Polyclonal antibody (27774-1-AP)

    WB analysis using 27774-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27774-1-AP (TRAPPC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TRAPPC11 Polyclonal antibody (27774-1-AP)

    IP experiment of HeLa using 27774-1-AP

    IP result of anti-TRAPPC11 (IP:27774-1-AP, 4ug; Detection:27774-1-AP 1:500) with HeLa cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TRAPPC11 Polyclonal antibody (27774-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27774-1-AP (TRAPPC11 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TRAPPC11 Polyclonal antibody (27774-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27774-1-AP (TRAPPC11 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TRAPPC11 Polyclonal antibody

TRAPPC11 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27774-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

C4orf41, UPF0636 protein C4orf41, Trafficking protein particle complex subunit 11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27774-1-AP targets TRAPPC11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAPPC11 fusion protein Ag27096 相同性解析による交差性が予測される生物種
Full Name chromosome 4 open reading frame 41
Observed molecular weight129 kDa
GenBank accession numberBC022185
Gene Symbol TRAPPC11
Gene ID (NCBI) 60684
RRIDAB_3085995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z392
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAPPC11 is a subunit of the TRAPP (transport protein particle) tethering complex, which functions in intracellular vesicle trafficking. This subunit is involved in early stage endoplasmic reticulum-to-Golgi vesicle transport. Mutations in TRAPPC11 result in neuromuscular and developmental phenotypes.

Protocols

Product Specific Protocols
WB protocol for TRAPPC11 antibody 27774-1-APDownload protocol
IHC protocol for TRAPPC11 antibody 27774-1-APDownload protocol
IP protocol for TRAPPC11 antibody 27774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols