Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, U-251 cells, NIH/3T3 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25282-1-AP targets TRAPPC2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRAPPC2L fusion protein Ag16890 相同性解析による交差性が予測される生物種 |
| Full Name | trafficking protein particle complex 2-like |
| Calculated molecular weight | 140 aa, 16 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC105809 |
| Gene Symbol | TRAPPC2L |
| Gene ID (NCBI) | 51693 |
| RRID | AB_2880006 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL33 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAPPC2L antibody 25282-1-AP | Download protocol |
| IHC protocol for TRAPPC2L antibody 25282-1-AP | Download protocol |
| IF protocol for TRAPPC2L antibody 25282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |