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TRAPPC3 Polyclonal antibody

Name: TRAPPC3 Polyclonal antibody
Brand: Proteintech
SKU: 15555-1-AP

TRAPPC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15555-1-AP

Synonyms

CDABP0066, BET3 homolog, BET3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 15555-1-AP
WB result of TRAPPC3 antibody (15555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC3 transfected HEK-293 cells.
WB analysis of mouse liver using 15555-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 15555-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15555-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 15555-1-AP
IP result of anti-TRAPPC3 (IP:15555-1-AP, 3ug; Detection:15555-1-AP 1:800) with mouse liver tissue lysate 4000ug.
IHC staining of human placenta using 15555-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15555-1-AP (TRAPPC3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 15555-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15555-1-AP (TRAPPC3 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 15555-1-AP
Immunofluorescent analysis of Hela cells, using TRAPPC3 antibody 15555-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 15555-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, PC-3 cells, HEK-293 cells, mouse small intestine tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below

Product Information

15555-1-AP targets TRAPPC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRAPPC3 fusion protein Ag7924 相同性解析による交差性が予測される生物種
Full Name	trafficking protein particle complex 3
Calculated molecular weight	20 kDa
Observed molecular weight	20-22 kDa
GenBank accession number	BC007662
Gene symbol	TRAPPC3
Gene ID (NCBI)	27095
RRID	AB_2208142
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRAPPC3 (trafficking protein particle complex 3, also known as Bet3) is a component of TRAPP, a complex involved in the tethering of transport vesicles to the cis-Golgi membrane. There are three TRAPP complexes identified in yeast with distinct roles: TRAPPI in ER-Golgi traffic, TRAPPII in intra-Golgi and endosome-Golgi traffic, and TRAPPIII in autophagy. Recently it has been proposed that at least two complexes exist in mammals. TRAPPC3 is the most conserved subunit of TRAPP and has been used to precipitate the intact tethering complex both from yeast and from human cells. It has also been reported that TRAPPC3 is required for Rabin8 centrosome trafficking and ciliogenesis. Expressed ubiquitously, TRAPPC3 protein is present in both membrane-bound and cytosolic forms. This antibody recognizes the endogenous 20-22 kDa TRAPPC3 in multiple cell lines. (15728249, 21273506, 23394947)

Protocols

Product Specific Protocols
WB protocol for TRAPPC3 antibody 15555-1-AP	Download protocol
IHC protocol for TRAPPC3 antibody 15555-1-AP	Download protocol
IF protocol for TRAPPC3 antibody 15555-1-AP	Download protocol
IP protocol for TRAPPC3 antibody 15555-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility. Authors - Bassik Michael C MC View Article
human	WB	Proc Natl Acad Sci U S A Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome. Authors - Westlake Christopher J CJ View Article
mouse	WB	Int J Biol Sci Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice Authors - Min Hu View Article
human	WB	J Biol Chem The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis. Authors - Adrian Cuenca View Article
human	WB	Signal Transduct Target Ther RING finger 138 deregulation distorts NF-кB signaling and facilities colitis switch to aggressive malignancy. Authors - Yalan Lu View Article
human	WB	Metabolites Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response Authors - Yun Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAPPC3 Polyclonal antibody

TRAPPC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 15555-1-AP

WB analysis of mouse liver using 15555-1-AP

WB analysis of mouse small intestine using 15555-1-AP

WB analysis of PC-3 using 15555-1-AP

IP experiment of mouse liver using 15555-1-AP

IHC staining of human placenta using 15555-1-AP

IHC staining of human placenta using 15555-1-AP

IF Staining of HeLa using 15555-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.

Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.

Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice

The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

RING finger 138 deregulation distorts NF-кB signaling and facilities colitis switch to aggressive malignancy.

Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response