Back

TRBP 抗体 13 文献

Rabbit Polyclonal| カタログ番号: 15753-1-AP

注目製品 KD/KO 検証済

  • Print page
  • Download PDF

--

（希望販売価格）

販売および在庫状況はコスモ・バイオ（株）にお問い合わせください。
distributor

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF
Application not tested? Please see our Guarantee


Images See all

TRBP Antibody WB HepG2 cells 15753-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TRBP Antibody IHC human brain tissue 15753-1-AP

Immunohistochemical analysis of paraffin-embedded human brain using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

TRBP Antibody IF HeLa cells 15753-1-AP

Immunofluorescent analysis of Hela cells, using TARBP2 antibody 15753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

TRBP Antibody IP mouse liver tissue 15753-1-AP

IP Result of anti-TRBP (IP:15753-1-AP, 4ug; Detection:15753-1-AP 1:1500) with mouse liver tissue lysate 6400ug.

種特異性:
human, mouse, rat
WB ポジティブ:
HepG2 cells, mouse liver tissue, mouse spleen tissue
IP ポジティブ:
mouse liver tissue
IHC ポジティブ:
human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
IF ポジティブ:
Hela cells
推奨希釈倍率:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
TRBP fusion protein Ag8255
正式名:
TAR (HIV-1) RNA binding protein 2
計算分子量:
39 kDa
検出分子量:
43-46 kDa
GenBank accession number:
BC005860
Gene ID (NCBI):
6895
遺伝子シンボル:
TARBP2
別名:
LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2

背景説明

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)


Back
to top