Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse liver tissue, mouse spleen tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 22 publications below |
| IF | See 2 publications below |
| IP | See 4 publications below |
| RIP | See 2 publications below |
Product Information
15753-1-AP targets TRBP in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRBP fusion protein Ag8255 相同性解析による交差性が予測される生物種 |
| Full Name | TAR (HIV-1) RNA binding protein 2 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 43-46 kDa |
| GenBank accession number | BC005860 |
| Gene Symbol | TRBP |
| Gene ID (NCBI) | 6895 |
| RRID | AB_2200485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRBP antibody 15753-1-AP | Download protocol |
| IHC protocol for TRBP antibody 15753-1-AP | Download protocol |
| IF protocol for TRBP antibody 15753-1-AP | Download protocol |
| IP protocol for TRBP antibody 15753-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.
| ||
Nat Commun Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells. | ||
Nucleic Acids Res S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.
| ||
J Med Chem Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma. |