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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRBP Monoclonal antibody (66792-1-Ig)

    WB analysis using 66792-1-Ig (same clone as 66792-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66792-1-Ig (TRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRBP Monoclonal antibody (66792-1-Ig)

    IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66792-1-Ig (TRBP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRBP Monoclonal antibody (66792-1-Ig)

    IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66792-1-Ig (TRBP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TRBP Monoclonal antibody, PBS Only

TRBP Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66792-1-PBS
Clone No.1C9A10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66792-1-PBS targets TRBP in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRBP fusion protein Ag8269 相同性解析による交差性が予測される生物種
Full Name TAR (HIV-1) RNA binding protein 2
Calculated molecular weight 39 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC005860
Gene Symbol TRBP
Gene ID (NCBI) 6895
RRIDAB_2882136
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15633
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)