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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human TREM-1/CD354 Rabbit Recombinant Antibody, PBS Only

TREM-1/CD354 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241279D10

Cat no : 98154-1-PBS

Synonyms

TREM1, TREM 1, CD354, 241279D10

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human TREM-1/CD354 Rabbit Recombinant Antibod (98154-1-RR)

    FC experiment of human peripheral blood leukocytes using 98154-1-RR (same clone as 98154-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TREM-1/CD354 Rabbit Recombinant Antibody (98154-1-RR, Clone: 241279D10) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98154-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human TREM-1/CD354 Rabbit Recombinant Antibod (98154-1-RR)

    FC experiment of human peripheral blood leukocytes using 98154-1-RR (same clone as 98154-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TREM-1/CD354 Rabbit Recombinant Antibody (98154-1-RR, Clone: 241279D10)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98154-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98154-1-RR

    Affinity and Kinetic Characterization of 98154-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98154-1-RR against Human TREM-1/CD354 were performed. The affinity constant is 0.676 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98154-1-PBS is TREM-1/CD354 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name triggering receptor expressed on myeloid cells 1
Calculated molecular weight26kDa
GenBank accession numberNM_018643.5
Gene symbol TREM1
Gene ID (NCBI) 54210
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Triggering receptor expressed on myeloid cells 1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.