Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| ELISA | See 1 publications below |
Product Information
27599-1-AP targets TREM2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26319 Product name: Recombinant human TREM2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-161 aa of BC032362 Sequence: HNTTVFQGVAGQSLQVSCPYDSMKHWGRRKAWCRQLGEKGPCQRVVSTHNLWLLSFLRRWNGSTAITDDTLGGTLTITLRNLQPHDAGLYQCQSLHGSEADTLRKVLVEVLADPLDHRDAGDLWFPGESESFEDAHVEHSISR 相同性解析による交差性が予測される生物種 |
| Full Name | triggering receptor expressed on myeloid cells 2 |
| Calculated molecular weight | 222 aa, 25 kDa |
| Observed molecular weight | 26-32 kDa |
| GenBank accession number | BC032362 |
| Gene Symbol | TREM2 |
| Gene ID (NCBI) | 54209 |
| RRID | AB_2880921 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZC2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).
Publications
| Species | Application | Title |
|---|---|---|
Nat Metab Inhibition of hepatic oxalate overproduction ameliorates metabolic dysfunction-associated steatohepatitis | ||
Nat Commun Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease | ||
Nat Commun A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution | ||
J Adv Res Luteolin as a novel therapeutic for diabetic kidney disease: Targeting the ADAM10-TREM2 pathway. | ||
J Clin Invest Inflammatory crosstalk impairs phagocytic receptors and aggravates atherosclerosis in clonal hematopoiesis in mice | ||
Cell Rep Med Targeting senescent EGR1(+) B cells enhances immunotherapy efficacy in esophageal squamous cell carcinoma. |