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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TREM2 Recombinant antibody

TREM2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240384B7

Cat no : 83438-6-RR

Synonyms

Trem2c, Trem2b, Trem2a, TREM-2, TREM 2

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using TREM2 Recombinant antibody (83438-6-RR)

    WB analysis of THP-1 using 83438-6-RR

    PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using TREM2 Recombinant antibody (83438-6-RR)

    WB analysis of THP-1 using 83438-6-RR

    Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83438-6-RR

    Affinity and Kinetic Characterization of 83438-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83438-6-RR against Human TREM2 were performed. The affinity constant is 38.7 nM.

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Tested Applications

Positive WB detected inPMA treated THP-1 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83438-6-RR is TREM2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name triggering receptor expressed on myeloid cells 2
Calculated molecular weight 222 aa, 25 kDa
Observed molecular weight26-32 kDa
GenBank accession numberBC032362
Gene symbol TREM2
Gene ID (NCBI) 54209
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).

Protocols

Product Specific Protocols
WB protocol for TREM2 antibody 83438-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols