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  • Featured Product
  • KD/KO Validated

TRIB2 Polyclonal antibody

TRIB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15359-1-AP

Synonyms

C5FW, GS3955, TRB 2, TRB2, TRIB2, Tribbles homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TRIB2 Polyclonal antibody (15359-1-AP)

    WB analysis of HeLa using 15359-1-AP

    WB result of TRIB2 antibody (15359-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIB2 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using TRIB2 Polyclonal antibody (15359-1-AP)

    WB analysis of HeLa using 15359-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    WB analysis of mouse thymus using 15359-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TRIB2 Polyclonal antibody (15359-1-AP)

    WB analysis of K-562 using 15359-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TRIB2 Polyclonal antibody (15359-1-AP)

    WB analysis of Jurkat using 15359-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human placenta using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human placenta using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human ovary using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human ovary using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human testis using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human testis using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human skin using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human skin using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human brain using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human brain using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human liver using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human liver using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human spleen using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human spleen using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human lung using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human lung using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human heart using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human heart using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human kidney using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TRIB2 Polyclonal antibody (15359-1-AP)

    IHC staining of human kidney using 15359-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse thymus tissue, K-562 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15359-1-AP targets TRIB2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIB2 fusion protein Ag7605 相同性解析による交差性が予測される生物種
Full Name tribbles homolog 2 (Drosophila)
Calculated molecular weight 39 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002637
Gene symbol TRIB2
Gene ID (NCBI) 28951
RRIDAB_2209083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIB2, also named as TRB2 , belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and Tribbles subfamily. TRIB2 interacts with MAPK kinases and regulates activation of MAP kinases. It does not display kinase activity. Antibodies against TRIB2 are present in sera from patients with autoimmune uveitis(uniprot). TRIB2 is enriched in hypocretin neurons. Sera from narcolepsy patients with cataplexy had higher Trib2-specific antibody titers compared with either normal controls or patients with idiopathic hypersomnia, multiple sclerosis, or other inflammatory neurological disorders.

Protocols

Product Specific Protocols
WB protocol for TRIB2 antibody 15359-1-APDownload protocol
IHC protocol for TRIB2 antibody 15359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IF

iScience

FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons

Authors - Wan Yun Ho
View Article
mouseWB,IF

Exp Mol Med

Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.

Authors - Eun Kyoung Do
  • KD Validated
View Article