Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 15359-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15359-1-AP
WB result of TRIB2 antibody (15359-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIB2 transfected HeLa cells.
WB analysis of Jurkat using 15359-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 15359-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 15359-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 15359-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

TRIB2 Polyclonal antibody

TRIB2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15359-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

C5FW, GS3955, TRB 2, TRB2, TRIB2, Tribbles homolog 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, mouse thymus tissue, K-562 cells
Positive IHC detected in	human placenta tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IF	See 2 publications below

Product Information

15359-1-AP targets TRIB2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIB2 fusion protein Ag7605 相同性解析による交差性が予測される生物種
Full Name	tribbles homolog 2 (Drosophila)
Calculated molecular weight	39 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC002637
Gene Symbol	TRIB2
Gene ID (NCBI)	28951
RRID	AB_2209083
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92519
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRIB2, also named as TRB2 , belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and Tribbles subfamily. TRIB2 interacts with MAPK kinases and regulates activation of MAP kinases. It does not display kinase activity. Antibodies against TRIB2 are present in sera from patients with autoimmune uveitis(uniprot). TRIB2 is enriched in hypocretin neurons. Sera from narcolepsy patients with cataplexy had higher Trib2-specific antibody titers compared with either normal controls or patients with idiopathic hypersomnia, multiple sclerosis, or other inflammatory neurological disorders.

Protocols

Product Specific Protocols
WB protocol for TRIB2 antibody 15359-1-AP	Download protocol
IHC protocol for TRIB2 antibody 15359-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IF	iScience FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons Authors - Wan Yun Ho View Article
mouse	WB,IF	Exp Mol Med Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency. Authors - Eun Kyoung Do KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15359-1-AP

WB analysis of HeLa using 15359-1-AP

WB analysis of Jurkat using 15359-1-AP

WB analysis of mouse thymus using 15359-1-AP

WB analysis of K-562 using 15359-1-AP

IHC staining of human placenta using 15359-1-AP

IHC staining of human placenta using 15359-1-AP

IHC staining of human testis using 15359-1-AP

IHC staining of human testis using 15359-1-AP

IHC staining of human skin using 15359-1-AP

IHC staining of human skin using 15359-1-AP

IHC staining of human brain using 15359-1-AP

IHC staining of human brain using 15359-1-AP

IHC staining of human liver using 15359-1-AP

IHC staining of human liver using 15359-1-AP

IHC staining of human spleen using 15359-1-AP