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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIB3 Monoclonal antibody, PBS Only

TRIB3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C10C7

Cat no : 66702-1-PBS

Synonyms

C20orf97, NIPK, SINK, SKIP3, TRB 3, TRB3, TRIB3, Tribbles homolog 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIB3 Monoclonal antibody (66702-1-Ig)

    WB analysis using 66702-1-Ig (same clone as 66702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRIB3 Monoclonal antibody (66702-1-Ig)

    WB analysis using 66702-1-Ig (same clone as 66702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using TRIB3 Monoclonal antibody (66702-1-Ig)

    WB analysis of rat liver using 66702-1-Ig (same clone as 66702-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse hepatocytes using TRIB3 Monoclonal antibody (66702-1-Ig)

    WB analysis of mouse hepatocytes using 66702-1-Ig (same clone as 66702-1-PBS)

    mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using TRIB3 Monoclonal antibody (66702-1-Ig)

    WB analysis of DU 145 using 66702-1-Ig (same clone as 66702-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66702-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using TRIB3 Monoclonal antibody (66702-1-Ig)

    WB analysis of LNCaP using 66702-1-Ig (same clone as 66702-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66702-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66702-1-PBS targets TRIB3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIB3 fusion protein Ag25776 相同性解析による交差性が予測される生物種
Full Name tribbles homolog 3 (Drosophila)
Calculated molecular weight 358 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC027484
Gene symbol TRIB3
Gene ID (NCBI) 57761
RRIDAB_2882055
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).