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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM11 Recombinant antibody (84651-3-RR)

    WB analysis using 84651-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84651-3-RR (TRIM11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84651-3-RR

    Affinity and Kinetic Characterization of 84651-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84651-3-RR against Human TRIM11 were performed. The affinity constant is 0.25 nM.

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TRIM11 Recombinant antibody

TRIM11 Recombinant Antibody for WB, ELISA
Cat No. 84651-3-RR
Clone No.242107F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RING finger protein 92, Protein BIA1, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM11, BIA1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, MCF-7 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84651-3-RR targets TRIM11 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM11 fusion protein Ag1308 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 11
Calculated molecular weight 52 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC011629
Gene Symbol TRIM11
Gene ID (NCBI) 81559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96F44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM11(Tripartite motif-containing protein 11) is also named as RNF92 and Belongs to the TRIM/RBCC family. It is a member of the tripartite-motif-containing protein family and is known to destabilize humanin, an inhibitor of Alzheimer-like neuronal insults(PMID: 16904669). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for TRIM11 antibody 84651-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols