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- KD/KO Validated
TRIM15 Polyclonal antibody
TRIM15 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IP, ELISA, IF
Conjugate
Unconjugated
Cat no : 13623-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
13623-1-AP targets TRIM15 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIM15 fusion protein Ag4533 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 15 |
| Calculated molecular weight | 465 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC038585 |
| Gene symbol | TRIM15 |
| Gene ID (NCBI) | 89870 |
| RRID | AB_2303892 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIM15 (Tripartite motif-containing protein 15) is also named as RNF93, ZNF178, ZNFB7 and belongs to the TRIM/RBCC family. It acts as an ubiquitin-protein ligase and contributes to the endogenous restriction of retroviruses in cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM15 antibody 13623-1-AP | Download protocol |
| IHC protocol for TRIM15 antibody 13623-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.
| ||
Biochim Biophys Acta Mol Basis Dis Ubiquitin ligase TRIM15 promotes the progression of pancreatic cancer via the upregulation of the IGF2BP2-TLR4 axis | ||
J Cell Sci TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly.
| ||
iScience Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma | ||
J Virol TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.
| ||
J Cancer Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses. |
