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  • Featured Product
  • KD/KO Validated

TRIM17 Polyclonal antibody

TRIM17 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13663-1-AP

Synonyms

tripartite motif containing 17, RNF16, RING-type E3 ubiquitin transferase TRIM17, RBCC, EC:2.3.2.27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM17 Polyclonal antibody (13663-1-AP)

    WB analysis using 13663-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13663-1-AP (TRIM17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TRIM17 Polyclonal antibody (13663-1-AP)

    WB analysis of HeLa using 13663-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13663-1-AP (TRIM17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TRIM17 Polyclonal antibody (13663-1-AP)

    WB analysis of COLO 320 using 13663-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13663-1-AP (TRIM17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIM17 Polyclonal antibody (13663-1-AP)

    IHC staining of human colon cancer using 13663-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13663-1-AP (TRIM17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIM17 Polyclonal antibody (13663-1-AP)

    IHC staining of human colon cancer using 13663-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13663-1-AP (TRIM17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using TRIM17 Polyclonal antibody (13663-1-AP)

    IF Staining of COLO 320 using 13663-1-AP

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using TRIM17 antibody (13663-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, mouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13663-1-AP targets TRIM17 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM17 fusion protein Ag4572 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 17
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC033788
Gene symbol TRIM17
Gene ID (NCBI) 51127
RRIDAB_10644443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRIM17 antibody 13663-1-APDownload protocol
IHC protocol for TRIM17 antibody 13663-1-APDownload protocol
IF protocol for TRIM17 antibody 13663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Differ

The E3 ubiquitin ligase TRIM17 promotes gastric cancer survival and progression via controlling BAX stability and antagonizing apoptosis

Authors - Jiajia Shen
  • KD Validated
View Article