Featured Product
KD/KO Validated

TRIM21 Monoclonal antibody

Name: TRIM21 Monoclonal antibody
Brand: Proteintech
SKU: 67136-1-Ig

TRIM21 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1E8B9

Review Product

Cat no : 67136-1-Ig

Synonyms

RNF 81, RING finger protein 81, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM21, 52 kDa ribonucleoprotein autoantigen Ro/SS-A

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 67136-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HeLa using 67136-1-Ig
WB result of TRIM21 antibody (67136-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM21 transfected HeLa cells.
WB analysis using 67136-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 67136-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 67136-1-Ig
HL-60 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 67136-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 67136-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 67136-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 67136-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 67136-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 67136-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 67136-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM21 antibody (67136-1-Ig, Clone: 1E8B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

-/ -

Cat No. 67136-1-Ig

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HL-60 cells, HSC-T6 cells, Jurkat cells, NIH/3T3 cells, NCI-H1299 cells, HepG2 cells, PC-12 cells
Positive IHC detected in	human breast cancer tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 9 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

67136-1-Ig targets TRIM21 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	TRIM21 fusion protein Ag28377 相同性解析による交差性が予測される生物種
Full Name	tripartite motif-containing 21
Calculated molecular weight	475 aa, 54 kDa
Observed molecular weight	42-54 kDa
GenBank accession number	BC010861
Gene symbol	TRIM21
Gene ID (NCBI)	6737
RRID	AB_2882435
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

Protocols

Product Specific Protocols
WB protocol for TRIM21 antibody 67136-1-Ig	Download protocol
IHC protocol for TRIM21 antibody 67136-1-Ig	Download protocol
IF protocol for TRIM21 antibody 67136-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Autophagy Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules Authors - Cuiwei Yang KD Validated View Article
human	WB	J Transl Med TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis Authors - Xiang-Lin Hao View Article
human	WB	Int J Mol Sci TRIM21 Promotes Rabies Virus Production by Degrading IRF7 through Ubiquitination Authors - Boyue Zhang KD Validated View Article
mouse	WB	Int Immunopharmacol TRIM21 deficiency confers protection from OGD/R-induced oxidative and inflammatory damage in cultured hippocampal neurons through regulation of the Keap1/Nrf2 pathway. Authors - Yahong Fu KD Validated View Article
human	IP,WB	Cell Death Dis RPRD1A stabilizes NRF2 and aggravates HCC progression through competing with p62 for TRIM21 binding. Authors - Xiaofan Feng View Article
human	WB	JCI Insight Alternative exon usage in TRIM21 determines the antigenicity of Ro52/TRIM21 in systemic lupus erythematosus Authors - Eduardo Gomez-Bañuelos View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM21 Monoclonal antibody

TRIM21 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 67136-1-Ig

WB analysis of HeLa using 67136-1-Ig

WB analysis using 67136-1-Ig

WB analysis of Jurkat using 67136-1-Ig

WB analysis of HL-60 using 67136-1-Ig

WB analysis of HSC-T6 using 67136-1-Ig

WB analysis of NIH/3T3 using 67136-1-Ig

IHC staining of human breast cancer using 67136-1-Ig

IHC staining of human pancreas cancer using 67136-1-Ig

IHC staining of human pancreas cancer using 67136-1-Ig

IHC staining of human breast cancer using 67136-1-Ig

IF Staining of HepG2 using 67136-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules

TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis

TRIM21 Promotes Rabies Virus Production by Degrading IRF7 through Ubiquitination

TRIM21 deficiency confers protection from OGD/R-induced oxidative and inflammatory damage in cultured hippocampal neurons through regulation of the Keap1/Nrf2 pathway.

RPRD1A stabilizes NRF2 and aggravates HCC progression through competing with p62 for TRIM21 binding.

Alternative exon usage in TRIM21 determines the antigenicity of Ro52/TRIM21 in systemic lupus erythematosus