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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM22 Recombinant antibody (83479-4-RR)

    WB analysis using 83479-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TRIM22 Recombinant antibody (83479-4-RR)

    WB analysis using 83479-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83479-4-RR (TRIM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TRIM22 Recombinant antibody (83479-4-RR)

    IF Staining of A431 using 83479-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRIM22 antibody (83479-4-RR, Clone: 240411D6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using TRIM22 Recombinant antibody (83479-4-RR)

    FC experiment of U2OS using 83479-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug TRIM22 Recombinant antibody (83479-4-RR, Clone:240411D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83479-4-RR

    Affinity and Kinetic Characterization of 83479-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83479-4-RR against Human TRIM22 were performed. The affinity constant is 0.181 nM.

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TRIM22 Recombinant antibody

TRIM22 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83479-4-RR
Clone No.240411D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

RING-type E3 ubiquitin transferase TRIM22, GPSTAF50, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, K-562 cells, A549 cells, U2OS cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83479-4-RR targets TRIM22 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM22 fusion protein Ag4683 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 22
Calculated molecular weight 498 aa, 57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC035582
Gene Symbol TRIM22
Gene ID (NCBI) 10346
RRIDAB_3671107
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IYM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid

Protocols

Product Specific Protocols
WB protocol for TRIM22 antibody 83479-4-RRDownload protocol
IF protocol for TRIM22 antibody 83479-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols