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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM24 Monoclonal antibody

TRIM24 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D6D9

Cat no : 66324-1-Ig

Synonyms

hTIF1, PTC6, RING finger protein 82, RNF82, TF1A, TIF1, TIF1 alpha, TIF1A, TIF1ALPHA, TRIM24, tripartite motif containing 24

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM24 Monoclonal antibody (66324-1-Ig)

    WB analysis using 66324-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TRIM24 Monoclonal antibody (66324-1-Ig)

    WB analysis of HeLa using 66324-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TRIM24 Monoclonal antibody (66324-1-Ig)

    WB analysis of HEK-293 using 66324-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human breast cancer using 66324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human lung cancer using 66324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human lung cancer using 66324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human liver cancer using 66324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human liver cancer using 66324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRIM24 Monoclonal antibody (66324-1-Ig)

    IHC staining of human breast cancer using 66324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66324-1-Ig targets TRIM24 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIM24 fusion protein Ag5417 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 24
Calculated molecular weight 117 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC028689
Gene symbol TRIM24
Gene ID (NCBI) 8805
RRIDAB_2881705
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) which is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

Protocols

Product Specific Protocols
WB protocol for TRIM24 antibody 66324-1-IgDownload protocol
IHC protocol for TRIM24 antibody 66324-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols