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TRIM26 Polyclonal antibody

Name: TRIM26 Polyclonal antibody
Brand: Proteintech
SKU: 27013-1-AP
Rating: 5.00 (1 reviews)

TRIM26 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 27013-1-AP

Synonyms

Acid finger protein, AFP, RING finger protein 95, RNF95, TRIM26, tripartite motif containing 26, Zinc finger protein 173, ZNF173

Validation Data Gallery

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AllWBIPIHC

WB analysis using 27013-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 27013-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 27013-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 27013-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 27013-1-AP
IP result of anti-TRIM26 (IP:27013-1-AP, 4ug; Detection:27013-1-AP 1:300) with HeLa cells lysate 2400ug.
IHC staining of human pancreas cancer using 27013-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 27013-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 27013-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 27013-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 27013-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 27013-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 27013-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, mouse testis tissue, RAW 264.7 cells, HeLa cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

27013-1-AP targets TRIM26 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIM26 fusion protein Ag25546 相同性解析による交差性が予測される生物種
Full Name	tripartite motif-containing 26
Calculated molecular weight	62 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC024039
Gene symbol	TRIM26
Gene ID (NCBI)	7726
RRID	AB_2880721
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRIM26 is a member of the tripartite motif (TRIM) protein family composed of more than 70 members in human. TRIM proteins share a similar characteristic structure, which includes a RING (R) domain, one or two B-boxes (B), and a coiled coil (CC) domain in the N-terminal and a domain in the C-terminal with variable structures. TRIM26 potentiates virus-triggered IRF3, NF-κB activation, IFN-β induction, and cellular antiviral responses. Further investigation suggests that TRIM26 plays an important role in the antiviral innate immune response by bridging TBK1 to NEMO and mediating TBK1 activation. (PMID: 25763818, PMID: 26611359)

Protocols

Product Specific Protocols
WB protocol for TRIM26 antibody 27013-1-AP	Download protocol
IHC protocol for TRIM26 antibody 27013-1-AP	Download protocol
IP protocol for TRIM26 antibody 27013-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma Authors - Zhangjie Wang View Article
pig	WB	Virus Res TRIM26-mediated degradation of nucleocapsid protein limits porcine reproductive and respiratory syndrome virus-2 infection. Authors - Pandeng Zhao View Article
mouse	WB	Cell Death Dis USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression Authors - Weiwei Wang View Article
human	WB,IHC,IF,IP	Int J Biol Sci TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation Authors - Yuening Sun KD Validated View Article
human	WB,IF	FASEB J TRIM26 restricts Epstein-Barr virus infection in nasopharyngeal epithelial cells through K48-linked ubiquitination of HSP-90β Authors - Mingjiao Zhang KD Validated View Article
human	WB	Aging (Albany NY) Machine learning-based identification and immune characterization of ferroptosis-related molecular clusters in osteoarthritis and validation Authors - Xiaocheng Guo View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Xingyuan (Verified Customer) (03-20-2024)

Protein quality was good.

Applications: Western Blot
Primary Antibody Dilution: 1:2500
Cell Tissue Type: HEK293T

TRIM26 Antibody Western Blot validation (1:2500 dilution) in HEK293T (Cat no:27013-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM26 Polyclonal antibody

TRIM26 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 27013-1-AP

WB analysis using 27013-1-AP

WB analysis of mouse testis using 27013-1-AP

WB analysis of RAW 264.7 using 27013-1-AP

IP experiment of HeLa using 27013-1-AP

IHC staining of human pancreas cancer using 27013-1-AP

IHC staining of human pancreas cancer using 27013-1-AP

IHC staining of human pancreas cancer using 27013-1-AP

IHC staining of human pancreas cancer using 27013-1-AP

IHC staining of human liver cancer using 27013-1-AP

IHC staining of human liver cancer using 27013-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma

TRIM26-mediated degradation of nucleocapsid protein limits porcine reproductive and respiratory syndrome virus-2 infection.

USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression

TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation

TRIM26 restricts Epstein-Barr virus infection in nasopharyngeal epithelial cells through K48-linked ubiquitination of HSP-90β

Machine learning-based identification and immune characterization of ferroptosis-related molecular clusters in osteoarthritis and validation

Reviews