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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM26 Recombinant antibody (84661-4-RR)

    WB analysis using 84661-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84661-4-RR (TRIM26 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84661-4-RR

    Affinity and Kinetic Characterization of 84661-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84661-4-RR against Human TRIM26 were performed. The affinity constant is 47.3 pM.

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TRIM26 Recombinant antibody

TRIM26 Recombinant Antibody for WB, ELISA
Cat No. 84661-4-RR
Clone No.242154B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZNF173, Tripartite motif-containing protein 26, TRIM, RNF95, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  100 μL, 1000 ug/ml
20 μL, 1000 ug/ml
100 μL, 1000 ug/ml

¥85,000/ 100 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84661-4-RR targets TRIM26 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM26 fusion protein Ag25546 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 26
Calculated molecular weight 62 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC024039
Gene Symbol TRIM26
Gene ID (NCBI) 7726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12899
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM26 is a member of the tripartite motif (TRIM) protein family composed of more than 70 members in human. TRIM proteins share a similar characteristic structure, which includes a RING (R) domain, one or two B-boxes (B), and a coiled coil (CC) domain in the N-terminal and a domain in the C-terminal with variable structures. TRIM26 potentiates virus-triggered IRF3, NF-κB activation, IFN-β induction, and cellular antiviral responses. Further investigation suggests that TRIM26 plays an important role in the antiviral innate immune response by bridging TBK1 to NEMO and mediating TBK1 activation. (PMID: 25763818, PMID: 26611359)

Protocols

Product Specific Protocols
WB protocol for TRIM26 antibody 84661-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols