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  • Featured Product
  • KD/KO Validated

TRIM27 Polyclonal antibody

TRIM27 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28751-1-AP

Synonyms

Ret finger protein, RFP, RING finger protein 76, RNF76, TRIM27, tripartite motif containing 27, Zinc finger protein RFP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM27 Polyclonal antibody (28751-1-AP)

    WB analysis using 28751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28751-1-AP (TRIM27 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIM27 Polyclonal antibody (28751-1-AP)

    IHC staining of human colon cancer using 28751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28751-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRIM27 Polyclonal antibody (28751-1-AP)

    IHC staining of human colon cancer using 28751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28751-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TRIM27 Polyclonal antibody (28751-1-AP)

    IHC staining of mouse testis using 28751-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28751-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH9).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TRIM27 Polyclonal antibody (28751-1-AP)

    IHC staining of mouse testis using 28751-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28751-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH9).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, Raji cells
Positive IHC detected inhuman colon cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28751-1-AP targets TRIM27 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM27 fusion protein Ag30576 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 27
Calculated molecular weight 513 aa, 58 kDa
Observed molecular weight58 kDa
GenBank accession numberBC013580
Gene symbol TRIM27
Gene ID (NCBI) 5987
RRIDAB_2881207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRIM27 antibody 28751-1-APDownload protocol
IHC protocol for TRIM27 antibody 28751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Lab Invest

TRIM27 contributes to glomerular endothelial cell injury in lupus nephritis by mediating the FoxO1 signaling pathway.

Authors - Jinxi Liu
  • KD Validated
View Article