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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIM27 Recombinant antibody (84927-6-RR)

    IF Staining of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRIM27 Recombinant antibody (84927-6-RR)

    IF Staining of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using TRIM27 Recombinant antibody (84927-6-RR)

    FC experiment of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TRIM27 Recombinant antibody (84927-6-RR)

    FC experiment of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84927-6-RR

    Affinity and Kinetic Characterization of 84927-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84927-6-RR against Human TRIM27 were performed. The affinity constant is 0.653 nM.

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TRIM27 Recombinant antibody, PBS Only

TRIM27 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84927-6-PBS
Clone No.242538H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Tripartite motif-containing protein 27, TRIM, RNF76, RFP, EC:2.3.2.27

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84927-6-PBS targets TRIM27 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM27 fusion protein Ag2849 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 27
Calculated molecular weight 513 aa, 58 kDa
GenBank accession numberBC013580
Gene Symbol TRIM27
Gene ID (NCBI) 5987
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP14373
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.