Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IP detected in | HT-29 cells |
| Positive IHC detected in | human small intestine tissue, Human Colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 17 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
Product Information
12543-1-AP targets TRIM31 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIM31 fusion protein Ag3264 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 31 |
| Calculated molecular weight | 425 aa, 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC017017 |
| Gene Symbol | TRIM31 |
| Gene ID (NCBI) | 11074 |
| RRID | AB_10640897 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZY9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIM31(Tripartite motif-containing protein 31) is also named as C6orf13, RNF and belongs to the TRIM/RBCC family. The deduced protein contains an N-terminal RING domain, a B-box-2 domain, and a coiled-coil region. TRIM31 forms homodimers(PMID:11331580). This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM31 antibody 12543-1-AP | Download protocol |
| IHC protocol for TRIM31 antibody 12543-1-AP | Download protocol |
| IP protocol for TRIM31 antibody 12543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity.
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Nat Commun USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS. | ||
Redox Biol The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR.
| ||
Cells Inhibition of Immunoproteasome Attenuates NLRP3 Inflammasome Response by Regulating E3 Ubiquitin Ligase TRIM31 | ||
J Virol TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity. |