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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM37 Polyclonal antibody (13037-1-AP)

    WB analysis using 13037-1-AP (same clone as 13037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRIM37 Polyclonal antibody (13037-1-AP)

    WB analysis using 13037-1-AP (same clone as 13037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRIM37 Polyclonal antibody (13037-1-AP)

    WB analysis using 13037-1-AP (same clone as 13037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TRIM37 Polyclonal antibody (13037-1-AP)

    IP experiment of mouse brain using 13037-1-AP (same clone as 13037-1-PBS)

    IP result of anti-TRIM37 (IP:13037-1-AP, 4ug; Detection:13037-1-AP 1:800) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TRIM37 Polyclonal antibody (13037-1-AP)

    IHC staining of mouse testis using 13037-1-AP (same clone as 13037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRIM37 Polyclonal antibody (13037-1-AP)

    IHC staining of human breast cancer using 13037-1-AP (same clone as 13037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TRIM37 Polyclonal antibody (13037-1-AP)

    IHC staining of mouse testis using 13037-1-AP (same clone as 13037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TRIM37 Polyclonal antibody (13037-1-AP)

    IF Staining of MCF-7 using 13037-1-AP (same clone as 13037-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using TRIM37 antibody (13037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using TRIM37 Polyclonal antibody (13037-1-AP)

    IF Staining of U-87 MG using 13037-1-AP (same clone as 13037-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using TRIM37 antibody (13037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13037-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TRIM37 Polyclonal antibody, PBS Only

TRIM37 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13037-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13037-1-PBS targets TRIM37 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM37 fusion protein Ag3690 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 37
Calculated molecular weight 964 aa, 108 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC036012
Gene Symbol TRIM37
Gene ID (NCBI) 4591
RRIDAB_10640800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94972
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TRIM37 (tripartite motif containing 37) is a member of the tripartite motif (RING, B-Box, and coiled-coil domains) family . Its gene mutations result in mulibrey nanism, which is a rare, autosomal-recessive growth disorder of prenatal onset, with characteristic dysmorphic features, pericardial constriction, and hepatomegaly .TRIM37 is located on chromosome 17q23, encoding a protein with an estimated molecular weight of 130 kDa or 89kDa. (PMID: 28098873, PMID: 29940807, PMID: 14757854)