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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM41 Polyclonal antibody

TRIM41 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18468-1-AP

Synonyms

tripartite motif containing 41, TRIM, RING finger-interacting protein with C kinase, RINCK, EC:2.3.2.27

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using TRIM41 Polyclonal antibody (18468-1-AP)

    WB analysis of BxPC-3 using 18468-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TRIM41 Polyclonal antibody (18468-1-AP)

    WB analysis of human placenta using 18468-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TRIM41 Polyclonal antibody (18468-1-AP)

    WB analysis of mouse liver using 18468-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TRIM41 Polyclonal antibody (18468-1-AP)

    WB analysis of human placenta using 18468-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TRIM41 Polyclonal antibody (18468-1-AP)

    WB analysis of mouse liver using 18468-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using TRIM41 Polyclonal antibody (18468-1-AP)

    IP experiment of human plasma using 18468-1-AP

    IP result of anti-TRIM41 (IP:18468-1-AP, 4ug; Detection:18468-1-AP 1:600) with human plasma lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TRIM41 Polyclonal antibody (18468-1-AP)

    IHC staining of human skeletal muscle using 18468-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18468-1-AP (TRIM41 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TRIM41 Polyclonal antibody (18468-1-AP)

    IHC staining of human skeletal muscle using 18468-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18468-1-AP (TRIM41 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRIM41 Polyclonal antibody (18468-1-AP)

    IHC staining of human breast cancer using 18468-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18468-1-AP (TRIM41 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRIM41 Polyclonal antibody (18468-1-AP)

    IHC staining of human breast cancer using 18468-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18468-1-AP (TRIM41 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using TRIM41 Polyclonal antibody (18468-1-AP)

    IF Staining of H9C2 using 18468-1-AP

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using TRIM41 antibody (18468-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inBxPC-3 cells, human placenta tissue, mouse liver tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman skeletal muscle tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inH9C2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18468-1-AP targets TRIM41 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM41 fusion protein Ag13236 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 41
Calculated molecular weight 72 kDa
Observed molecular weight 70-72 kDa
GenBank accession numberBC018765
Gene symbol TRIM41
Gene ID (NCBI) 90933
RRIDAB_10859823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRIM41 antibody 18468-1-APDownload protocol
IHC protocol for TRIM41 antibody 18468-1-APDownload protocol
IF protocol for TRIM41 antibody 18468-1-APDownload protocol
IP protocol for TRIM41 antibody 18468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Heliyon

IDI1 inhibits the cGAS-Sting signaling pathway in hepatocellular carcinoma

Authors - Lin Fu
View Article