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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM43 Polyclonal antibody (31053-1-AP)

    WB analysis using 31053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31053-1-AP (TRIM43 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using TRIM43 Polyclonal antibody (31053-1-AP)

    FC experiment of A549 using 31053-1-AP

    1x10^6 A549 cells were intracellularly stained with 0.4 ug TRIM43 Polyclonal antibody (31053-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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TRIM43 Polyclonal antibody

TRIM43 Polyclonal Antibody for WB, ELISA
Cat No. 31053-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Tripartite motif-containing protein 43, TRIM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, A549 cells, Jurkat cells, U-87 MG cells, mouse brain tissue
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31053-1-AP targets TRIM43 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM43 fusion protein Ag34926 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 43
Observed molecular weight52-60 kDa
GenBank accession numberBC015353
Gene Symbol TRIM43
Gene ID (NCBI) 129868
RRIDAB_3669834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BQ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM43 (tripartite motif containing 43), also known as TRIM43A. It is expected to be located in the cytoplasm. The calculated molecular weight of TRIM43 is 52 kDa. TRIM43 was also upregulated in herpesvirus-associated diseases in humans, including γ herpesvirus-associated tumors. As such, TRIM43 and its transcription factor DUX4 could potentially serve as biomarkers of active herpesviral replication and pathogenesis (PMID: 30420784).

Protocols

Product Specific Protocols
WB protocol for TRIM43 antibody 31053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols