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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TRIM44 Monoclonal antibody (66249-1-Ig)

    WB analysis of MCF-7 using 66249-1-Ig (same clone as 66249-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66249-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using TRIM44 Monoclonal antibody (66249-1-Ig)

    WB analysis of human testis using 66249-1-Ig (same clone as 66249-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66249-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TRIM44 Monoclonal antibody (66249-1-Ig)

    IF Staining of MCF-7 using 66249-1-Ig (same clone as 66249-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66249-1-Ig(TRIM44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66249-1-PBS in a different storage buffer formulation.

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TRIM44 Monoclonal antibody, PBS Only

TRIM44 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66249-1-PBS
Clone No.1B12H12

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66249-1-PBS targets TRIM44 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TRIM44 fusion protein Ag18771 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 44
Calculated molecular weight 344 aa, 38 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC024031
Gene Symbol TRIM44
Gene ID (NCBI) 54765
RRIDAB_2881637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96DX7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TRIM44 is one of the family member of TRIM protein, which contains an N-terminal ubiquitin hydrolase-type zinc-finger domain, followed by a coiled-coil domain, a zinc-finger B-box homology domain, and a second coiled-coil domain near the C-terminus. Members of the TRIM protein family are involved in various cellular processes, such as cell proliferation, oncogenesis and antiviral defense.