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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM46 Recombinant antibody, PBS Only (Capture)

TRIM46 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240557E1

Cat no : 83614-2-PBS

Synonyms

Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM46 Recombinant antibody (83614-2-RR)

    WB analysis using 83614-2-RR (same clone as 83614-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using TRIM46 Recombinant antibody (83614-2-RR)

    WB analysis of U-87 MG using 83614-2-RR (same clone as 83614-2-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TRIM46 Recombinant antibody (83614-2-RR)

    IF Staining of A549 using 83614-2-RR (same clone as 83614-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM46 antibody (83614-2-RR, Clone: 240557E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TRIM46 Recombinant antibody (83614-2-RR)

    FC experiment of HeLa using 83614-2-RR (same clone as 83614-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRIM46 Recombinant antibody (83614-2-RR, Clone:240557E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00591-1

    Cytometric bead array standard curve of MP00591-1

    Cytometric bead array standard curve of MP00591-1, TRIM46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83614-2-PBS. Detection antibody: 83614-4-PBS. Standard: Ag15290. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00591-2

    Cytometric bead array standard curve of MP00591-2

    Cytometric bead array standard curve of MP00591-2, TRIM46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83614-2-PBS. Detection antibody: 83614-3-PBS. Standard: Ag15290. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83614-2-RR

    Affinity and Kinetic Characterization of 83614-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83614-2-RR against Human TRIM46 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83614-2-PBS targets TRIM46 as part of a matched antibody pair:

MP00591-1: 83614-2-PBS capture and 83614-4-PBS detection (validated in Cytometric bead array)

MP00591-2: 83614-2-PBS capture and 83614-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM46 fusion protein Ag15290 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 46
Calculated molecular weight 759 aa, 83 kDa
Observed molecular weight83 kDa
GenBank accession numberBC069416
Gene symbol TRIM46
Gene ID (NCBI) 80128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).