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  • Featured Product
  • KD/KO Validated

TRIM5 Polyclonal antibody

TRIM5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11476-1-AP

Synonyms

RING finger protein 88, RNF88, TRIM5, TRIM5alpha, tripartite motif containing 5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TRIM5 Polyclonal antibody (11476-1-AP)

    WB analysis of HeLa using 11476-1-AP

    WB result of TRIM5 antibody (11476-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM5 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using TRIM5 Polyclonal antibody (11476-1-AP)

    WB analysis using 11476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TRIM5 Polyclonal antibody (11476-1-AP)

    WB analysis using 11476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using TRIM5 Polyclonal antibody (11476-1-AP)

    WB analysis of mouse stomach using 11476-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRIM5 Polyclonal antibody (11476-1-AP)

    IHC staining of mouse liver using 11476-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11476-1-AP (TRIM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRIM5 Polyclonal antibody (11476-1-AP)

    IHC staining of mouse liver using 11476-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11476-1-AP (TRIM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using TRIM5 Polyclonal antibody (11476-1-AP)

    IHC staining of human stomach using 11476-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11476-1-AP (TRIM5 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, MKN-45 cells
Positive IHC detected inmouse liver tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11476-1-AP targets TRIM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM5 fusion protein Ag1973 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 5
Calculated molecular weight 493 aa, 56 kDa
Observed molecular weight 46 kDa, 56 kDa
GenBank accession numberBC021258
Gene symbol TRIM5
Gene ID (NCBI) 85363
RRIDAB_2877767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM5 belongs to the large tripartite motif protein family, members of which typically are composed of 3 zinc-binding domains, a RING, unique B-box type 1 and B-box type 2 domains, followed by a coiled-coil (CC) region. TRIM proteins use homomultimerization to identify specific cell compartments. TRIM5 promotes innate immune signaling and that this activity is amplified by retroviral infection and interaction with the capsid lattice. TRIM5 has 6 variants termed alpha, beta, gamma, delta, epsilon, and iota with the MW of 56 kDa, 46 kDa, 40 kDa, 37 kDa, 31 kDa and 29 kDa. TRIM5 can form homodimers (~70 kDa) and homotrimers (90-160 kDa).

Protocols

Product Specific Protocols
WB protocol for TRIM5 antibody 11476-1-APDownload protocol
IHC protocol for TRIM5 antibody 11476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols